ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (10): 1616-1621.doi: 10.11843/j.issn.0366-6964.2014.10.006

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Analysis on the Genetic Diversity of Wenshang Barred Chicken Breed

DU Yan-zhi1,2,CHEN Yu-xia1,KANG Li1,XU Lai-xiang2,TANG Hui1,JIANG Yun-liang1*   

  1. (1.Laboratory of Animal Molecular Genetics,College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai’an 271018,China;2.College of Life Sciences,Qufu Normal University,Qufu 273165,China)
  • Received:2014-01-25 Online:2014-10-23 Published:2014-10-23

Abstract:

In this study,one hundred chickens including 60 hens and 40 roosters were sampled for the evaluation of genetic diversity on Wenshang Barred chicken breed by using 27 pairs of primers recommended jointly by FAO (Food and Agriculture) and ISAG (the International Society for Animal Genetic).The genetic diversity at DNA level were analyzed in this study.Totally 86 alleles were detected,the number of alleles varied from 2 to 6,giving a mean value of 3.185 alleles per locus.The average heterozygosity and polymorphism information content of 27 microsatellite loci were 0.465 5 and 0.402 3,respectively.The genetic distances between 3 populations distributed in different areas vary from 0.05 to 0.10.The results indicated that Wenshang Barred chicken breed had low heterozygosity and polymorphism information content,which imply that effective methods should be taken to protect this breed.

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